How to Make Potato Dextrose Agar (PDA) Using Potato Flakes

Potato flakes are an excellent choice for preparing Potato Dextrose Agar (PDA), a widely used medium for agar plates!

Key Properties of PDA Agar Mix:

• Sterilizes at temperatures above 121°C for 90 minutes per 250ml
• Melts at temperatures over 80°C
• Solidifies at temperatures below -45°C

Ingredients for 1000ml of PDA Agar:

• 2000ml water jug (for mixing)
• 1000ml boiling water (distilled water is recommended for best results)
• 20g potato flakes
• 15g agar powder
• 10g cane sugar, maple syrup, or regular sugar
• 5-6 drops of agar colorant (We use blue as it helps highlight contamination more effectively.)

Preparation Method:

1. Combine all ingredients in the water jug, stirring thoroughly until you achieve a smooth, consistent mixture. The smoother, the better!
2. Transfer the mixture into sterilization-safe bottles or containers.
3. Before placing the container in a pressure cooker, ensure the lid is loosely secured to prevent pressure buildup and potential spills.
4. Sterilize at 121°C for 90 minutes to ensure a contaminant-free medium.
5. Once sterilized, the PDA mix can be stored for future use.

⚠️ Safety Note: Always exercise caution when handling boiling water to prevent burns. We are not responsible for any mishandling or accidents that may occur.

What you will receive

You will receive 100g of potato flakes ready for all your agar needs.

How To Use Agar

Check out this handy help sheet if you need help with your Agar!

Disclaimer

Please note that it is illegal to grow magic mushrooms within the UK. We ask that you do not use these spores to grow mushrooms, but instead use them to study under a microscope. We cannot be held liable for any illegal use of these spores. Please check your local regulations as we cannot be responsible for the laws you should follow in your own country.

Contact Us

Please don’t hesitate to contact us if you have any questions or concerns regarding your order! You can call, email, or message us between Monday to Friday, 9-5 (excluding bank holidays).